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polarity nylon hybridization transfer membrane  (Beyotime)


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    Structured Review

    Beyotime polarity nylon hybridization transfer membrane
    Polarity Nylon Hybridization Transfer Membrane, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polarity+nylon+hybridization+transfer+membrane/polarity+nylon+hybridization+membrane/pmc07734107-104-22-27
    Average 90 stars, based on 1 article reviews
    polarity nylon hybridization transfer membrane - by Bioz Stars, 2026-09
    90/100 stars

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    Nucleic Acid Electrophoresis:

    Article Title: Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice
    Article Snippet: The nuclear proteins (5 μg) of each group were incubated with 1 × binding buffer (LightShift Chemiluminescent EMSA Kit, Pierce) in the presence of 50 ng/μl poly (dI/dC), 0.05% non-idet P-40, 5 mM MgCl 2 , and 2.5% glycerol for 10 min and then incubated at room temperature for additional 20 min with 1 pmol of biotin-labeled Ap-1 oligonucleotide (Sangon Biotech Co., Ltd.). .. The reaction mixture was subjected to a 6% non-denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 100 v for 50 min, transferred to polarity nylon hybridization transfer membrane (Beyotime, China) and DNA cross-linked for 2 min, and probed with peroxidase-conjugated streptavidin antibodies (1:1,500 dilution, Bytotime, China), then visualized with enhanced chemiluminescence and detected by ChemiDoc RXC + (Bio-Rad, United States). ..

    Hybridization:

    Article Title: Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice
    Article Snippet: The nuclear proteins (5 μg) of each group were incubated with 1 × binding buffer (LightShift Chemiluminescent EMSA Kit, Pierce) in the presence of 50 ng/μl poly (dI/dC), 0.05% non-idet P-40, 5 mM MgCl 2 , and 2.5% glycerol for 10 min and then incubated at room temperature for additional 20 min with 1 pmol of biotin-labeled Ap-1 oligonucleotide (Sangon Biotech Co., Ltd.). .. The reaction mixture was subjected to a 6% non-denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 100 v for 50 min, transferred to polarity nylon hybridization transfer membrane (Beyotime, China) and DNA cross-linked for 2 min, and probed with peroxidase-conjugated streptavidin antibodies (1:1,500 dilution, Bytotime, China), then visualized with enhanced chemiluminescence and detected by ChemiDoc RXC + (Bio-Rad, United States). ..

    Membrane:

    Article Title: Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice
    Article Snippet: The nuclear proteins (5 μg) of each group were incubated with 1 × binding buffer (LightShift Chemiluminescent EMSA Kit, Pierce) in the presence of 50 ng/μl poly (dI/dC), 0.05% non-idet P-40, 5 mM MgCl 2 , and 2.5% glycerol for 10 min and then incubated at room temperature for additional 20 min with 1 pmol of biotin-labeled Ap-1 oligonucleotide (Sangon Biotech Co., Ltd.). .. The reaction mixture was subjected to a 6% non-denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 100 v for 50 min, transferred to polarity nylon hybridization transfer membrane (Beyotime, China) and DNA cross-linked for 2 min, and probed with peroxidase-conjugated streptavidin antibodies (1:1,500 dilution, Bytotime, China), then visualized with enhanced chemiluminescence and detected by ChemiDoc RXC + (Bio-Rad, United States). ..



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    90
    Beyotime polarity nylon hybridization transfer membrane
    Polarity Nylon Hybridization Transfer Membrane, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polarity+nylon+hybridization+transfer+membrane/polarity+nylon+hybridization+membrane/pmc07734107-104-22-27
    Average 90 stars, based on 1 article reviews
    polarity nylon hybridization transfer membrane - by Bioz Stars, 2026-09
    90/100 stars
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